Gene

TwTPS21

Gene Family

diterpene synthases

Species

Tripterygium wilfordii

Sequence Length

4005bp

Accession Number

H265Y

Reaction Equation

Sequence

atgcggggttctcatcatcatcatcatcatggtatggctagcatgactggtggacagcaaatgggtcgggatctgtacgacgatgacgataaggatccgaattcgagctcccatatgccgttcgtaaacaaacagttcaactataaagacccagtcaacggcgtggacattgcctatatcaaaatcccgaatgcgggtcaaatgcagcccgtgaaagcatttaaaatccataacaaaatttgggtgatcccggagcgcgatacgttcacgaacccggaagaaggagatttaaacccaccgcctgaggctaaacaggtcccggtgtcttactatgatagcacatacctgagtaccgacaatgaaaaggacaactacctgaaaggtgttaccaaactgttcgagcgcatttattcgacagatctcggtcgcatgttgctgacttctattgtgcgcggcattccgttttggggtggtagcaccatcgatacagaactcaaagtgattgacaccaactgcatcaatgtgattcagcctgatgggagctaccggtccgaagagcttaacctcgtaatcattggcccgagcgcggatattatccaattcgaatgtaaatcttttgggcatgaagtcctgaatctgacgcggaatggctatggatcgacgcagtatattcgtttttctccagatttcacatttggatttgaagaaagcctcgaagttgatacgaaccctcttttaggcgcgggaaaattcgcgacggacccagcggtgaccttggcacatgaacttatttatgccgggcatcgcttgtatggaatcgccattaacccgaaccgtgttttcaaggtgaatacgaacgcgtattacgagatgtcgggcttagaagtgtcctttgaagaactgcgcacgtttggcggtcatgatgcaaaatttattgatagtctgcaagaaaacgaatttcggctgtactattacaataaattcaaagacattgcatcaaccttaaacaaggcgaaaagcattgtgggtaccacggctagcttacaatatatgaaaaacgttttcaaagaaaaatacctccttagcgaagacacttccggcaaattctctgtcgataaactgaaatttgataaactgtataaaatgctcaccgagatctacacagaggataactttgtcaaattcttcaaggtcttgaatcggaaaacctatctgaacttcgataaagccgtctttaagatcaacatcgtaccgaaagttaactacaccatctatgatggctttaatctgcgcaatacgaatctggcggcgaactttaacggccagaacaccgaaatcaacaacatgaactttactaaactgaaaaattttaccggcttgtttgaattttataagctcctgtgtgtccgcggtattatcaccagcaaaaccaaatccttggataagggctataacaaggcgctcaatgatttatgcatcaaggtgaacaactgggacttgtttttctctccatctgaagataattttactaacgacttgaacaaaggagaggaaattacttccgataccaacatcgaagcagcggaagagaatattagcctggatcttattcaacaatattacctgacctttaattttgataacgagcctgagaacatttccattgagaatctcagctctgacatcatcggccagctggaactgatgccgaatatcgaacgctttcctaatggaaagaaatatgaattggacaaatacaccatgttccactatctccgcgcgcaggagtttgagcacggcaagtctcgtattgctctgaccaattcggtaaacgaagcccttttaaatccttcgcgtgtgtacacctttttctcaagcgattatgttaaaaaagtgaacaaggcgaccgaagcggcgatgtttttgggatgggtggaacaactggtatatgactttacggatgaaacttctgaagtctcgaccaccgacaaaattgccgatattaccattatcattccctatattggccctgcactgaacattggtaacatgctgtataaagatgattttgtgggcgccctgatcttttcaggcgctgttatcctgctggaatttatcccggaaatcgccattccagtactcggtacctttgcgctggtgtcctatatcgcaaacaaagttttgactgtccagacgatcgacaacgcgctcagtaaacgtaacgaaaaatgggatgaggtgtataagtatattgttaccaactggctcgctaaagtaaacacccagattgacctgattcgcaagaagatgaaagaagcgctggaaaaccaagcagaagcgaccaaagctattatcaactatcaatataaccagtacacagaggaagaaaagaataacatcaacttcaacatcgacgacttatcttcaaagctgaatgaatctattaacaaagcgatgattaatattaacaagttcttgaaccaatgtagtgtcagctatctgatgaactcgatgatcccatatggtgtgaaacgtctggaagacttcgatgcaagccttaaagatgcccttctgaagtatatttacgataatcgcggaactcttattggccaagtggatcgcttaaaagataaagtcaacaacacgctgagtacagacatcccttttcagctgtctaaatatgtggacaatcagcgcctgctgtccacgtttacggaatacatcaaaaacatcatcaacactagtattctgaacttgcgttacgagagtaaccatctgattgatctgagccgttacgcatctaaaatcaacatcggctcgaaggtgaacttcgatcctatcgacaaaaaccagattcaattgttcaacttagaatcgtcaaagattgaagttatcttaaaaaatgcgattgtatataattcaatgtacgaaaatttctctacgagcttttggattcgtattccgaaatatttcaacagtatctctttaaacaacgagtatactatcatcaattgtatggagaataacagcgggtggaaagtgagccttaactatggtgaaatcatctggactctgcaggacactcaagaaattaaacaacgcgtggtgtttaaatactcacagatgattaacatctcggattatattaatcgctggatttttgtgacaattactaacaaccggctgaacaacagcaaaatttacattaacggtcgcctgatcgatcagaaaccaatcagtaatctcggtaacattcacgcatcgaataatatcatgttcaaactggatggttgtcgcgacacgcaccgttacatttggatcaaatacttcaatttattcgacaaagaactcaacgaaaaggagattaaggatctttatgacaatcagtctaattcgggtattctgaaagacttttggggtgattaccttcagtacgataaaccgtattatatgttaaacttatatgatccgaataaatacgttgacgtcaacaacgttggcattcgtggctatatgtatctgaaagggccgcgtggcagcgtgatgaccactaacatttacttaaactcctccctctatcgcggtactaaatttattatcaagaaatatgcctctggcaacaaggacaatatcgtacgcaataacgatcgcgtctacattaacgtggtggtgaagaataaagaatatcgtctggcgaccaatgctagtcaggcgggcgtggagaaaattctgtctgcacttgaaatcccggatgtgggtaatttatcccaggtggttgtgatgaaaagtaaaaatgaccaagggatcaccaataaatgcaaaatgaatctgcaagataacaacggcaacgacattggttttatcggcttccaccaattcaataatatcgcgaaactggtggcctcaaattggtacaaccgtcagattgagcgcagctcccgcactttaggctgtagctgggagttcattccggtagatgacggttggggagaacgcccattgtaa

Reference

Hansen N L, Nissen J N, Hamberger B. Two residues determine the product profile of the class II diterpene synthases TPS14 and TPS21 of Tripterygium wilfordii[J]. Phytochemistry, 2017, 138: 52-56.