PrimerServer

Define Regions

Species

Region Type

Target Region

Include Region

Force 3' End

Product Size

Min bp

Max bp

Setting Primer Design Parameters

Primer3 Settings

Primer Size (bp):

Min Opt Max

Primer G+C (%):

Min Opt Max

Primer Tm (°C):

Min Opt Max

Primer Pair Tm Max Diff. (°C):

No. Primers To Return:

3' End Distance (bp) Between:

Left Primers Right Primers

Max 3' Stability:

Lowercase masking:

Yes

Thermodynamic Secondary Structure Alignments Setting

TH: Max Self Complementarity (°C):

TH: Max Pair Complementarity (°C):

TH: Max 3' Self Complementarity (°C):

TH: Max 3' Pair Complementarity (°C):

TH: Max Primer Hairpin (°C):

Input Primer Sequences (5' to 3')

Recent Saved Results

	Time	Title	Status	URL

Setting Specificity Checking Parameters

Setting Filter Parameters

Checking Prodcut Size (bp):

Min Max

Min. Tm Difference:

(°C) lower

Max. Primers Return Number:

Filter with 3' End Validation:

Yes

Max. Amplicons Return Per Primer:

Setting BLAST

E-value:

Word Size:

Min. Identity (%):

Max. HSPs:

Setting Experimental Conditions

Concentration of primers (nM):

Concentration of dNTPs (mM):

Concentration of Na⁺ (mM):

Concentration of K⁺ (mM):

Concentration of Tris (mM):

Concentration of Mg²⁺ (mM):

Select Checking Databases

Checking Databases

Selected Databases

PrimerServer: PCR Primers Batch Design & Specificity Check

Setting Primer Design Parameters

Recent Saved Results

Setting Specificity Checking Parameters